Detection and sequencing of Potato virus Y (PVY) and Potato leafroll virus (PLRV) in a volunteer plant of Solanum tuberosum L. cv. Diacol-Capiro
نویسندگان
چکیده
منابع مشابه
Digoxigenin-labelled molecular probe for the simultaneous detection of three potato pathogens: potato spindle tuber viroid (PSTVd), potato virus Y (PVY), and potato leafroll virus (PLRV).
A molecular probe, p3POT, was constructed of PSTVd, PVY, PLRV cDNA fragments introduced into pUC18 vector. Sequencing of the inserts revealed that cloned fragments covered conservative parts of pathogenic genomes. Dot-blot hybridization of digoxigenin-labelled construct to crude extracts from plants infected with different potato viruses proved high sensitivity and specificity of the p3POT prob...
متن کاملComparison between Proteome and Transcriptome Response in Potato (Solanum tuberosum L.) Leaves Following Potato Virus Y (PVY) Infection
Plant diseases caused by viral infection are affecting all major crops. Being an obligate intracellular organisms, chemical control of these pathogens is so far not applied in the field except to control the insect vectors of the viruses. Understanding of molecular responses of plant immunity is therefore economically important, guiding the enforcement of crop resistance. To disentangle complex...
متن کاملInvolvement of Potato (Solanum tuberosum L.) MKK6 in Response to Potato virus Y
Mitogen-activated protein kinase (MAPK) cascades have crucial roles in the regulation of plant development and in plant responses to stress. Plant recognition of pathogen-associated molecular patterns or pathogen-derived effector proteins has been shown to trigger activation of several MAPKs. This then controls defence responses, including synthesis and/or signalling of defence hormones and act...
متن کاملتعیین برخی خصوصیات بیولوژیکی و مولکولی potato leafroll virus و potato virus a در استان اصفهان
چکیده سیب زمینی یکی از مهم ترین محصولات زراعی در جهان می باشد. ویروس های متعددی این گیاه را تحت تاثیر قرار می دهند و باعث خسارت اقتصادی قابل توجهی می شوند. ویروس پیچیدگی برگ سیب زمینی potato leafroll virus (plrv) عضو تیپ جنس polerovirus و خانواده luteoviridae و ویروس ای سیب زمینی potato virus a (pva) عضو تیپ جنس potyvirus از خانواده potyviridae شایع ترین ویروس های سیب زمینی می باشند که توسط ش...
15 صفحه اولResistance to Potato virus Y (PVY) in potato cv. Pito transformed with the P1 gene of PVY
ACADEMIC DISSERTATION IN PLANT PATHOLOGY To be presented with the permission of the Faculty of Agriculture and Forestry of the University of Helsinki for public criticism in Viikki, Auditorium B2, on June 30th, at 12 o'clock noon. HELSINKI 2000 2 Mäki-Valkama, T. Resistance to Potato virus Y (PVY) in potato cv. Pito transformed with the P1 gene of PVY. Doctoral thesis in plant pathology. 43 p. ...
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ژورنال
عنوان ژورنال: Acta Agronómica
سال: 2017
ISSN: 2323-0118,0120-2812
DOI: 10.15446/acag.v66n4.59753